Related Experiment Video
Updated: Aug 8, 2026

Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells
Published on: July 21, 2009
Immunocytochemistry in effusion cytology: a contemporary review
1Cytopathology Section, National Institutes of Health/National Cancer Institute, Bethesda, Maryland 20892-1500, USA.
Background:
Cytology plays a pivotal role in the diagnosis of pleural effusions. In many cases, immunocytochemistry (ICC) is required to elucidate the etiology of the atypical cells. Effusions are samples that present unique problems for ICC. To date there is no standardization of ICC methods for effusions and cytology in general.
Methods:
The authors review the most commonly used cytologic preparations, fixatives, and antibodies used in effusion ICC.
Results:
Through the utilization of cell block preparations and a panel of antibodies appropriate for the differential diagnosis in question, ICC conditions utilized in surgical pathology can be most closely replicated.
Conclusions:
ICC may provide reliable insights into various diagnostic dilemmas in effusion cytology, provided that laboratory standardization practices are followed.
More Related Videos
07:43Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
Published on: July 29, 2017
07:52Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Published on: January 9, 2019
Related Concept Videos
Flow Cytometry
In...
Immunofluorescence Microscopy
The...
Immunocytochemistry and Immunohistochemistry
These...
Confocal Fluorescence Microscopy