Related Experiment Video
Updated: Oct 3, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
Operational modes for transient isotachophoretic preconcentration-capillary zone electrophoresis and detectable
T Hirokawa1, H Okamoto, N Ikuta
1Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University, Higashi-hiroshima, Japan. hiro77@hiroshima-u.ac.jp
Abstract:
Operational modes for transient isotachophoretic preconcentration capillary zone electrophoresis (tr-ITP-CZE) were studied by using 5 microM and 0.5 microM rare earth mixtures as analytes in comparison with field-enhanced sample stacking. After examination of several operational modes for tr-ITP, it was found that tr-ITP effectively occured even if both the leading electrolyte and the terminating electrolyte were injected after the sample plug. This was explained as the result of a field-enhanced stacking for both sample components and the leading and terminating ions. The observed theoretical plate numbers were 4-20 times higher than those obtained by normal stacking; and the estimated low limit of detectable concentration of rare-earth elements (REE) was ca. 0.1 microM which was 2.5 times lower compared to normal stacking. For the 0.5 microM sample, a concentration factor of 20 000 could be achieved after only tr-ITP.
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Ion-Exchange Chromatography
Capillary Electrophoresis: Instrumentation
Electrophoresis: Overview
There...
High-Performance Liquid Chromatography: Types of Detectors
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
