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Updated: Aug 7, 2026
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
Identification of EBV transforming genes by recombinant EBV technology
1Department of Microbiology, Harvard Medical School and Medicine, Brigham and Women's Hospital, 857 Channing Laboratory, 181 Longwood Ave, Boston, MA 02115, USA. izumi@uthscsa.edu
Abstract:
Epstein-Barr virus (EBV) is able to infect primary B-lymphocytes but usually does not proceed to replicate more virions. Instead, EBV persists as an incomplete virus and expresses 12 gene products that transform the growth of these cells into continuously proliferating lymphoblastoid cell lines. Because EBV is associated with several human malignancies, there is intense interest in delineating the molecular functions of these EBV gene products in transformation. This review focuses on the recombinant EBV technologies that have been developed to introduce specific mutations into EBV and test the functions of these EBV genes in primary B-lymphocyte growth transformation.
