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Evaluation of transplanted hepatocytes using HIDA scintigraphy
1Department of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi.
Summary
In vivo 99mTc-HIDA scintigraphy effectively assessed hepatocyte graft function after intrasplenic transplantation in rats. This imaging technique shows promise for monitoring transplanted liver cells.
Area of Science:
- Hepatology
- Transplantation Biology
- Medical Imaging
Background:
- Hepatocyte transplantation offers potential for treating liver diseases.
- Assessing the function of transplanted hepatocytes is crucial for clinical success.
- Previous studies utilized ex-vivo HIDA scintigraphy for graft evaluation.
Purpose of the Study:
- To evaluate the efficacy of in-vivo 99mTc-HIDA scintigraphy.
- To assess hepatocyte graft function following intrasplenic transplantation.
- To determine if HIDA scintigraphy can monitor transplanted cell viability.
Main Methods:
- Rat hepatocytes were isolated using collagenase digestion.
- Hepatocytes were transplanted intrasplenically into syngenic rats.
- In-vivo 99mTc-HIDA scintigraphy and histology were performed 90 days post-transplantation.
Main Results:
- Five out of six transplanted rats demonstrated prompt HIDA uptake in the spleen.
- Histology confirmed the presence of hepatocytes within the splenic red pulp.
- Control rats receiving saline showed no splenic HIDA accumulation.
Conclusions:
- In-vivo 99mTc-HIDA scintigraphy is a viable method for assessing hepatocyte graft function.
- This imaging modality can monitor the viability and function of intrasplenically transplanted hepatocytes.
- HIDA scintigraphy may aid in the clinical management of hepatocyte transplantation.