Cloning of 87 kDa outer membrane protein gene of Pasteurella multocida P52

Pasteurella multocida serotype B:2 is the causative agent of haemorrhagic septicaemia, a fatal disease of cattle and buffaloes. Formalin inactivated whole cell bacterin is used to prepare vaccines in India. However, outer membrane proteins (OMPS) of P. multocida were reported to be potential immunogens. The 87-kDa OMP of P. multocida P52, serotype B:2 has been identified as one of the major antigens because this protein reacted with serum of vaccinated animals. The gene omp87, encoding an 87-kDa OMP was amplified and cloned into pBluescript SK(-) vector. This gene was found to localise in a 9.0 kb Hind III fragment of P. multocida genome.