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Related Experiment Videos

Nanoliter-scale non-invasive flow-rate quantification using micro-interferometric back-scatter and phase detection.

D A Markov1, D J Bornhop

  • 1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409-1061, USA.

Fresenius' Journal of Analytical Chemistry
|October 27, 2001
PubMed
Summary

Researchers developed a non-invasive method to measure fluid velocity in tiny capillaries. This technique uses laser heating and a special detector to accurately quantify flow rates in microfluidic devices.

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Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Optical Sensing

Background:

  • Miniaturization of fluid-handling systems, especially microfluidic devices, enhances chemical and biochemical analyses.
  • Accurate, non-invasive measurement of flow velocity in nano- and picoliter volumes remains challenging.

Purpose of the Study:

  • To present a simple, non-invasive method for detecting and measuring linear flow velocity in fluid-filled capillaries.
  • To enable precise flow rate quantification in microscale fluidic systems.

Main Methods:

  • Utilized local heating of a small fluid volume with an infrared laser diode.
  • Employed a micro-interferometric back-scatter detector (MIBD) downstream to detect thermally induced refractive index changes.
  • Calculated fluid velocity by analyzing the phase difference between the heating function and MIBD output in the Fourier domain.

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Main Results:

  • Quantified flow rates between 1 and 10 µL/min within a 40 nL probe volume.
  • Achieved a 3-sigma detection limit of 42.8 nL/min for flow velocity measurements.
  • Demonstrated a non-invasive approach for microscale fluid dynamics analysis.

Conclusions:

  • The presented method offers a viable solution for accurate flow velocity measurement in microfluidic channels.
  • This technique supports the advancement of miniaturized analytical systems requiring precise fluid control.
  • The non-invasive nature and sensitivity make it suitable for various microfluidic applications.