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Related Experiment Videos

Electrical activity in embryonic heart cell aggregates. Developmental aspects.

T F McDonald, H G Sachs

    Pflugers Archiv : European Journal of Physiology
    |January 1, 1975
    PubMed
    Summary
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    Embryonic chick heart cells in aggregates show developing action potential parameters, with fast inward channels appearing and slow channels shifting function. Aggregate cultures mimic intact tissue membrane properties.

    Area of Science:

    • Cardiology
    • Developmental Biology
    • Electrophysiology

    Background:

    • Embryonic heart cells undergo significant electrophysiological changes during development.
    • Understanding these changes is crucial for studying cardiac development and disease.

    Purpose of the Study:

    • To investigate the development of action potential parameters in embryonic chick heart cell aggregates.
    • To characterize the ionic channel mechanisms underlying these developmental changes.

    Main Methods:

    • Cultured embryonic chick heart cells (2.5, 4, and 7 days) in aggregate form.
    • Measured action potential parameters including maximum diastolic potential, overshoot, V max, and duration.
    • Utilized pharmacological agents like TTX and D600 to probe ionic channel activity.

    Related Experiment Videos

  • Examined the effects of adrenaline on action potentials.

    • Main Results:

    • Action potential parameters (maximum diastolic potential, overshoot, V max, duration) increased with age in whole heart aggregates.
    • Action potential duration varied between atrial, whole heart, and ventricular aggregates (atrial < whole heart < ventricular).
    • Early aggregates (2.5 days) relied on slow channels (blocked by D600), while later aggregates (7 days) showed functional fast channels (blocked by TTX).
    • Adrenaline restored action potential parameters in D600-treated 7-day aggregates.

    Conclusions:

    • Embryonic heart cell action potential development is marked by the emergence of fast inward channels.
    • The slow channel mechanism transitions from primary action potential generation to a role in the plateau phase.
    • Embryonic heart cells in aggregate cultures exhibit membrane properties similar to intact cardiac tissue.