Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatographic Methods: Terminology

Chromatographic Methods: Terminology

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...

Column Efficiency: Rate Theory

Column Efficiency: Rate Theory

The rate theory of chromatography provides quantitative insight into the shapes and widths of elution bands. These bands are based on the random-walk mechanism governing molecular migration within a column. The Gaussian profile of chromatographic bands arises from the cumulative effect of random molecular motions as they progress through the column.
During elution, a solute molecule experiences numerous transitions between stationary and mobile phases, exhibiting irregular residence times in...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Applications of molecularly imprinted polymers to the analysis and removal of personal care products: A review.

Talanta·2015

Same author

Apoptotic cell-derived ICAM-3 promotes both macrophage chemoattraction to and tethering of apoptotic cells.

Cell death and differentiation·2011

Same author

A charge coupled device array detector for single-wavelength and multiwavelength ultraviolet absorbance in capillary electrophoresis.

Analytical chemistry·2011

Same author

Systematic Approach to Links between Separations in MEKC and Reversed-Phase HPLC.

Analytical chemistry·2011

Same author

Quantification of organic acids in beer by nuclear magnetic resonance (NMR)-based methods.

Analytica chimica acta·2010

Same author

[Tuberculosis cutis colliquativa. Even today an important differential diagnosis].

Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete·2009

Search research articles

Related Experiment Video

Updated: Jul 12, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Predicting peak shape in capillary zone electrophoresis: a generic approach to parametrizing peaks using the

G L Erny¹, E T Bergström, D M Goodall

¹Department of Chemistry, University of York, UK.

Analytical Chemistry

|October 30, 2001

Summary

The Haarhoff-Van der Linde (HVL) peak function accurately models capillary zone electrophoresis (CZE) peaks, even with electromigration dispersion (EMD). This model helps quantify peak distortion and improve variance analysis in CZE.

More Related Videos

Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets

Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets

Published on: February 10, 2020

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Related Experiment Videos

Last Updated: Jul 12, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets

Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets

Published on: February 10, 2020

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Area of Science:

Analytical Chemistry
Separation Science

Background:

Capillary Zone Electrophoresis (CZE) is a powerful separation technique.
Electromigration Dispersion (EMD) significantly impacts peak shape and resolution in CZE.
Existing models often fail to accurately describe distorted CZE peaks.

Purpose of the Study:

To evaluate the Haarhoff-Van der Linde (HVL) peak function for modeling CZE peaks.
To quantify peak distortion caused by EMD in CZE.
To develop a more accurate method for analyzing peak variance in CZE.

Main Methods:

Application of the Haarhoff-Van der Linde (HVL) peak function to CZE data.
Utilizing moving boundary theory to relate peak distortion parameters to experimental conditions.
Derivation of a universal function and master equation for HVL peak variance.

Main Results:

The HVL function effectively fits distorted CZE peaks, accounting for both Gaussian and triangular peak characteristics.
A peak distortion parameter was derived, linked to analyte/background electrolyte properties and electric field.
EMD significantly contributes to peak variance, especially under typical CZE conditions.

Conclusions:

The HVL model provides a superior method for analyzing CZE peak shapes and variances compared to simple additive models.
Accurate quantification of EMD effects is crucial for optimizing CZE separations.
The developed methodology enables better prediction and control of peak broadening in CZE.