Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Melatonin radioimmunoassay.

J Arendt, L Paunier, P C Sizonenko

    The Journal of Clinical Endocrinology and Metabolism
    |February 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    A new radioimmunoassay for melatonin was developed using rabbit antibodies. This method is specific and more sensitive than the tadpole bioassay for melatonin detection.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Circadian rhythms and sleep regulation in seasonal affective disorder.

    Acta neuropsychiatrica·2016
    Same author

    Why get big in the cold? Size-fecundity relationships explain the temperature-size rule in a pulmonate snail (Physa).

    Journal of evolutionary biology·2014
    Same author

    [A few reflections and definitions on the subject of embryonic research].

    Bulletin de la Societe des sciences medicales du Grand-Duche de Luxembourg·2013
    Same author

    Pharmacokinetics of melatonin in preterm infants.

    British journal of clinical pharmacology·2013
    Same author

    [Can one authorize oocyte donation in the Grand Duchy of Luxembourg?].

    Bulletin de la Societe des sciences medicales du Grand-Duche de Luxembourg·2012
    Same author

    An hour of bright white light in the early morning improves performance and advances sleep and circadian phase during the Antarctic winter.

    Neuroscience letters·2012
    Same journal

    Continuous glucose monitoring-derived time in range is associated with changes in arterial stiffness in type 2 diabetes.

    The Journal of clinical endocrinology and metabolism·2026
    Same journal

    Association of the Primary Aldosteronism Severity Classification with Lateralization and Treatment Outcomes.

    The Journal of clinical endocrinology and metabolism·2026
    Same journal

    From Premature Adrenarche to Adult Metabolic Risk and Hyperandrogenism: A Systematic Review and Meta-Analysis.

    The Journal of clinical endocrinology and metabolism·2026
    Same journal

    Open-Label 9-Year Follow-Up Extension Phase 2 Study of Once-Weekly Somatrogon in Children With Growth Hormone Deficiency.

    The Journal of clinical endocrinology and metabolism·2026
    Same journal

    Correction to: "CAHQL: A Patient-Reported Outcome Instrument to Assess Health-Related Quality of Life in Congenital Adrenal Hyperplasia".

    The Journal of clinical endocrinology and metabolism·2026
    Same journal

    Approach to Personalizing the Treatment of Osteoporosis.

    The Journal of clinical endocrinology and metabolism·2026
    See all related articles

    Area of Science:

    • Endocrinology
    • Immunology
    • Assay Development

    Background:

    • Melatonin is a crucial hormone regulating circadian rhythms.
    • Accurate measurement of melatonin is essential for physiological and clinical studies.
    • Existing bioassays for melatonin have limitations in sensitivity and specificity.

    Purpose of the Study:

    • To develop and validate a novel radioimmunoassay (RIA) for quantifying melatonin.
    • To compare the performance of the developed RIA with existing melatonin detection methods.

    Main Methods:

    • Generation of specific anti-melatonin antibodies in rabbits.
    • Development of a radioimmunoassay protocol utilizing these antibodies.
    • Validation of the RIA's specificity and sensitivity.

    Related Experiment Videos

    Main Results:

    • Successful development of a specific radioimmunoassay for melatonin.
    • The developed RIA demonstrated a greater sensitivity range compared to the traditional tadpole bioassay.
    • The assay showed high specificity for melatonin.

    Conclusions:

    • The developed radioimmunoassay provides a sensitive and specific tool for melatonin measurement.
    • This RIA offers an improved alternative to the tadpole bioassay for melatonin quantification.
    • The new assay has potential applications in research and diagnostics involving melatonin.