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Two mechanisms activate PTPalpha during mitosis.

X M Zheng1, D Shalloway

  • 1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.

The EMBO Journal
|November 2, 2001
PubMed
Summary
This summary is machine-generated.

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Protein tyrosine phosphatase alpha (PTPalpha) activates Src during mitosis through serine hyperphosphorylation. This phosphatase uniquely activates Src, a process crucial for cell cycle progression.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Protein tyrosine phosphatase alpha (PTPalpha) plays a role in cell signaling.
  • Src activation is critical for cell cycle progression, particularly during mitosis.

Purpose of the Study:

  • To elucidate the mechanisms by which PTPalpha is activated during mitosis.
  • To determine the specific role of PTPalpha in the activation of Src during mitosis.

Main Methods:

  • Analysis of PTPalpha activity and its interaction with Grb2 during mitosis.
  • Investigation of Src activation in PTPalpha-knockout cells during mitosis.
  • Assessment of the combined effects of PTPalpha and Cdc2 on Src activation.

Main Results:

Related Experiment Videos

  • PTPalpha activation during mitosis is dependent on serine hyperphosphorylation, increasing its specific activity and decreasing its binding to Grb2.
  • PTPalpha facilitates Src dephosphorylation by abating Grb2 inhibition and transiently binds to Src.
  • Src is not activated in mitosis in PTPalpha-knockout cells, highlighting PTPalpha's unique mitotic role.

Conclusions:

  • PTPalpha is the key serine phosphorylation-activated, membrane-bound protein tyrosine phosphatase that activates Src during mitosis.
  • Mitotic activation of Src is quantitatively explained by PTPalpha activation and Cdc2-mediated Src phosphorylations.