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Updated: Jul 16, 2026
17:14
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
Published on: December 10, 2012
Developmental regulation of DNA replication timing at the human beta globin locus
I Simon1, T Tenzen, R Mostoslavsky
1Department of Cellular Biochemistry, Hebrew University Medical School, Jerusalem, Israel.
The EMBO Journal
|November 2, 2001
Summary
DNA replication timing of the human beta globin locus is controlled by the locus control region. This timing is established independently of gene transcription, offering insights into gene regulation.
Area of Science:
- Genetics
- Molecular Biology
- Epigenetics
Background:
- The human beta globin locus exhibits cell-type-specific DNA replication timing.
- It replicates late in non-erythroid cells but early in erythroid cells.
Purpose of the Study:
- To investigate the regulatory mechanisms controlling DNA replication timing at the beta globin locus.
- To determine if regulatory elements within the locus control region (LCR) influence replication timing.
- To ascertain the relationship between replication timing and gene transcription.
Main Methods:
- Fluorescence in situ hybridization (FISH) to map DNA replication timing.
- Genetic analysis in transgenic mouse models.
Main Results:
- Regulatory elements within the locus control region (LCR) control both late and early replication states.
- Replication timing patterns are established independently of gene transcription.
Conclusions:
- The locus control region (LCR) is a key determinant of DNA replication timing at the human beta globin locus.
- Replication timing is regulated by mechanisms distinct from transcriptional control.
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