Vascular endothelial growth factor mRNA synthesis by peripheral blood mononuclear cells in patients with acute
Y Sasaki1, A Kawamoto, M Iwano
1First Department of Internal Medicine, Nara Medical University, 840 Shijo, Kashihara, 634-8522, Japan.
Abstract:
We studied vascular endothelial growth factor (VEGF) mRNA synthesis by peripheral blood mononuclear cells (PBMCs) in 30 patients with acute myocardial infarction (AMI) and 20 healthy individuals. PBMCs were isolated from all patients on days 3 and 14 after the onset of aMI, and from all of control individuals. To prepare samples containing identical amounts of GAPDH cDNA, competitive PCR was performed by co-amplifying serial dilutions of GAPDH mutant templates. Next, to measure VEGF cDNA quantitatively in the samples containing identical amounts of GAPDH, we also used competitive PCR by co-amplifying mutant templates of VEGF. The serum VEGF concentrations on day 14 in patients with aMI were measured by an ELISA method. Higher levels of VEGF mRNA in PBMCs were present on day 14 than either on day 3 or in the control group. Serum VEGF concentrations correlated with the VEGF mRNA levels of PBMCs on day 14. Peak serum CK levels correlated well with VEGF mRNA levels of PBMCs on day 14. The present findings suggest that PBMCs may be one of the candidates responsible for elevated circulatory VEGF protein following aMI. In addition, VEGF mRNA may be overexpressed in PBMCs in response to cardiac muscle damage.
Insights
Peripheral blood mononuclear cells (PBMCs) showed higher vascular endothelial growth factor (VEGF) mRNA levels after acute myocardial infarction (AMI). These findings suggest PBMCs contribute to elevated circulatory VEGF post-AMI.
Area of Science:
- Cardiovascular Biology
- Molecular Biology
- Immunology
Background:
- Acute myocardial infarction (AMI) is a critical condition associated with significant cardiovascular events.
- Vascular Endothelial Growth Factor (VEGF) plays a crucial role in angiogenesis and tissue repair.
- Understanding the source of elevated VEGF in AMI is vital for therapeutic strategies.
Purpose of the Study:
- To investigate VEGF mRNA synthesis in peripheral blood mononuclear cells (PBMCs) of AMI patients.
- To correlate PBMC VEGF mRNA levels with serum VEGF concentrations and cardiac damage markers.
Main Methods:
- Quantitative analysis of VEGF mRNA in PBMCs using competitive Polymerase Chain Reaction (PCR).
- Measurement of serum VEGF concentrations via ELISA.
- Inclusion of healthy controls and serial sampling (days 3 and 14 post-AMI).
Main Results:
- VEGF mRNA levels in PBMCs were significantly higher on day 14 compared to day 3 and controls.
- Serum VEGF concentrations on day 14 positively correlated with PBMC VEGF mRNA levels.
- Peak serum Creatine Kinase (CK) levels correlated with elevated PBMC VEGF mRNA on day 14.
Conclusions:
- Peripheral blood mononuclear cells (PBMCs) are a likely source of increased circulatory VEGF following acute myocardial infarction.
- VEGF mRNA is overexpressed in PBMCs in response to cardiac muscle damage, suggesting a role in the post-AMI response.
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