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Updated: Jul 24, 2026
12:41
Stress-induced Antibiotic Susceptibility Testing on a Chip
Published on: January 8, 2014
Does stress influence ampicillin concentration in serum and tissues?
C Tesseromatis1, A Trichilis, E Tsivos
1Department of Pharmacology, Medical School, University of Athens, Greece.
European Journal of Drug Metabolism and Pharmacokinetics
|November 7, 2001
Summary
Consecutive exercise-induced stress in rats increased plasma ampicillin levels. This finding suggests that physiological stress from exercise can alter drug concentrations, impacting therapeutic efficacy and requiring further investigation in clinical settings.
Area of Science:
- Pharmacology
- Exercise Physiology
- Biochemistry
Background:
- Exercise alters drug levels and increases free fatty acids (FFAs).
- FFAs can interfere with drug-protein binding due to high albumin affinity.
- This interaction may affect drug efficacy and safety.
Purpose of the Study:
- To evaluate the influence of consecutive exercise-induced stress on ampicillin levels in Wistar rats.
- To investigate the relationship between physiological stress markers and ampicillin pharmacokinetics.
Main Methods:
- Two groups of Wistar rats were used: experimental (Group A) and control (Group B).
- Group A underwent cold swimming stress for varying durations (5-30 days).
- Animals received intramuscular ampicillin injections; plasma levels and stress parameters were measured.
Main Results:
- Exercise-induced stress significantly increased FFAs, adrenal weight, and hematocrit (Ht%) in experimental groups.
- Ampicillin levels were elevated in all experimental groups compared to the control group.
- A positive correlation was observed between stress parameters and ampicillin concentration.
Conclusions:
- Consecutive exercise-induced stress leads to increased ampicillin levels in rats.
- Physiological stress, mediated by FFAs, appears to influence ampicillin pharmacokinetics.
- These findings highlight the importance of considering exercise and stress in drug level monitoring.
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