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Published on: March 27, 2014
Determination of isoflavone glucoside malonates in Trifolium pratense L. (red clover) extracts: quantification and
E De Rijke1, A Zafra-Gómez, F Ariese
1Department of Analytical Chemistry and Applied Spectroscopy, Free University, Amsterdam, The Netherlands.
Abstract:
Isoflavones, their glucosides and their glucoside malonates were determined in red clover leaf extracts using reversed-phase LC coupled to atmospheric pressure chemical ionisation mass spectrometry (APCI-MS), UV and fluorescence detectors and the stability of the malonates was investigated. Extracts can be stored at least 1-2 weeks at -20 degrees C without loss of malonates. In LC-separated fractions the malonates are most stable when stored at low temperature after evaporation to dryness. The concentrations of eight major isoflavones ranged from 0.04 to 5 mg/g leaves.
