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Updated: Aug 8, 2026
Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
The prognostic significance of day 3 embryo cleavage stage on subsequent blastocyst development in a sequential
R Boostanfar1, J K Jain, C C Slater
1Department of Obstetrics and Gynecology, Women's and Children's Hospital, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA. drboostanfar@hotmail.com
Purpose:
To determine prognostic significance of blastomere number on Day 3 of culture upon subsequent blastocyst (BL) development.
Methods:
A retrospective analysis was conducted in 37 IVF subjects undergoing standard protocols and BL transfer after sequential embryo culture in P1 and BL media.
Results:
Of Day 3 embryos containing 7 or more blastomeres, 68.9% (186/270) developed into BL compared to embryos containing 4-6 blastomeres, 38.1% (56/147), P < 0.0001. The majority of BL, 68.9% (168/244), were observed on Day 5. Extended Day 6 culture represented 31.1% (76/244) of all BLs.
Conclusions:
The observation of 7 or more blastomeres on Day 3 yielded a significantly greater likelihood of BL development. Embryos containing 4-6 blastomeres are still relatively likely to progress to a BL. Extended culture to Day 6 still yields a significant proportion of BL. Cell cleavage stage on Day 3 appears to be a useful prognostic indicator of subsequent BL development.
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