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Exon 2 sequence analysis of a novel HLA-DRB1 allele, DRB1*0314
H J Koninkx1, H J Tijssen, I Joosten
1Department of Blood Transfusion and Transplantation Immunology, University Medical Center Nijmegen, 6500 NB Nijmegen, The Netherlands.
Tissue Antigens
|November 13, 2001
Abstract:
We describe the identification of a new DRB1*03 variant in a Caucasian volunteer bone-marrow donor (DR'KW'). The indication for the existence of this new variant arose from contradictory results obtained by serological and several DNA typing methods (SSP, SSOP). Analysis of the exon 2 sequence revealed that the DRB1*0314 contained the characteristic DR3 specific sequence motifs, except for codon 77 (AAC-->ACC) where threonine is substituted for asparagine.