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Intricacies in ATP-dependent clamp loading: variations across replication systems
1Department of Chemistry, 414 Wartik Laboratory, Pennsylvania State University, University Park, PA 16802, USA.
Abstract:
DNA replication requires the coordinated effort of many proteins to create a highly processive biomachine able to replicate entire genomes in a single process. The clamp proteins confer on replisomes this property of processivity but in turn require clamp loaders for their functional assembly onto DNA. A more detailed view of the mechanisms for holoenzyme assembly in replication systems has been obtained from the advent of novel solution experiments and the appearance of low- and high-resolution structures for the clamp loaders.
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