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Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
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Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
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Related Experiment Video

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Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
08:14

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Published on: February 25, 2017

Dynamic protein interactions in the bacteriophage T4 replisome.

M A Trakselis1, M U Mayer, F T Ishmael

  • 1Dept of Chemistry, 414 Wartik Laboratory, The Pennsylvania State University, University Park, PA 16802, USA.

Trends in Biochemical Sciences
|September 12, 2001
PubMed
Summary

The bacteriophage T4 DNA replisome

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • The bacteriophage T4 DNA replisome is a complex molecular machine responsible for DNA replication.
  • Its function relies on the precise assembly and interaction of numerous protein components.
  • Understanding these dynamics is crucial for comprehending DNA synthesis mechanisms.

Purpose of the Study:

  • To elucidate the dynamic structural and kinetic assembly of the bacteriophage T4 DNA polymerase holoenzyme.
  • To investigate the formation of the primosome, a key complex in DNA replication initiation.
  • To model the transient biomolecular interactions governing replisome assembly.

Main Methods:

  • Utilized small molecules, including fluorescent probes and crosslinkers, to dissect protein interactions.
  • Developed a dynamic structural and kinetic model.
  • Focused on the assembly of DNA synthesis and priming protein complexes.

Main Results:

  • Detailed a complex dynamic model for DNA polymerase holoenzyme assembly.
  • Characterized the intricate interplay of proteins during primosome formation.
  • Provided insights into the transient biomolecular interactions critical for replication.

Conclusions:

  • The bacteriophage T4 DNA replisome assembly is a highly coordinated process involving transient protein interactions.
  • The developed model offers a framework for understanding the dynamic nature of DNA replication machinery.
  • This research enhances our knowledge of the structural and kinetic aspects of DNA synthesis and primosome formation.