Related Experiment Video
Updated: Aug 11, 2026
09:42
Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
Published on: June 19, 2012
SsrA-Based Targeted Protein Degradation in Escherichia coli
Qichang Nie1, Terrence Chi-Kong Lau2
1Department of Biomedical Sciences, College of Biomedicine, City University of Hong Kong, Kowloon, Hong Kong, SAR, China.
Methods in Molecular Biology (Clifton, N.J.)
|August 10, 2026
Summary
Bacterial PROTACs offer a novel approach to combat antimicrobial resistance by targeting drug-resistant proteins like CTX-M-14. This method resensitizes bacteria to antibiotics, reviving their efficacy.
Area of Science:
- Drug discovery and development
- Molecular biology
- Antimicrobial resistance research
Background:
- Targeted protein degradation is a promising drug discovery strategy.
- Proteolysis-targeting chimeras (PROTACs) harness the proteasome to degrade proteins.
- Antimicrobial resistance (AMR) is a growing global health threat, driven by drug-resistant bacteria.
Purpose of the Study:
- To develop a novel bacterial PROTAC (BacPROTAC) system.
- To target and degrade the drug-resistant CTX-M-14 β-lactamase.
- To resensitize AMR bacteria to antibiotics.
Main Methods:
- Utilized an ssrA-based system to create bacterial PROTACs.
- Applied BacPROTACs to target CTX-M-14 in AMR bacteria.
- Assessed the resensitization of bacteria to antibiotics.
Main Results:
- Successfully developed and applied ssrA-based BacPROTACs.
- Demonstrated the degradation of CTX-M-14, a key AMR determinant.
- Showed potential for resensitizing drug-resistant bacteria.
Conclusions:
- Bacterial PROTACs represent a viable strategy for combating AMR.
- This approach can revive the effectiveness of existing antibiotics.
- Opens new therapeutic avenues for treating infections caused by AMR bacteria.
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