Related Experiment Videos
Deglycosylated mucin as a substrate in enzymatic O-glycosylation in vitro
A Paszkiewicz-Gadek1, H Porowska, D Kisiel
1Department of General and Organic Chemistry, Medical Academy of Białystok.
Roczniki Akademii Medycznej W Bialymstoku (1995)
|November 20, 2001
Abstract:
To obtain the apomucin as a substrate for glycosyltransferases activity testing, the pig gastric mucin deglycosylation by chemical method was carried out. Resulted apomucin, rich in Ser, Thr and Pro residues, was as good carbohydrate acceptor in enzymatic O-glycosylation in vitro, as synthetic peptide, analogue of MUC2 mucin tandem repeats sequence.