An mRNA degrading complex in Rhodobacter capsulatus
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Updated: Aug 12, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Published on: January 16, 2017
1Institut für Mikro- und Molekularbiologie, Justus Liebig Universität Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Abstract:
An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation, and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.
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