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[Effects of endogenous TGF-beta during wound healing]
1Burn Center, Changhai Hospital, Second Military Medical University, Shanghai 200433.
Objective:
To study the application of exogenous TGF-beta to excision, incision and burn wounds accelerating wound healing.
Methods:
The expression of endogenous TGF-beta during burn wound healing was observed by mRNA dot blot hybridization and immunohistochemistry, respectively.
Results:
Thermal injury could induce TGF-beta gene expression in cutaneous wounds. Expression level of TGF-beta mRNA increased over time to reach a peak around day 5 postburn, and declined at day 10 after thermal injury. Immunohistochemistry evidences indicated spatially and temporally that TGF-beta peptide mainly distributed at hair follicles and migrating epidermis at early and middle stages of repair process, and stronger immunoreactivity from days 5 to 10 after wound, which was directly proportional to epidermal cell migration and dermal fibroblast proliferation.
Conclusion:
The expression models of TGF-beta protein and gene suggests that TGF-beta play a critical role in regulation of repair process. Whether the inhibition of expression and overexpression of TGF-beta is correlated to imparied repair and scar formation remains to be studied.