Related Experiment Video
Updated: Aug 6, 2026

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
Published on: July 6, 2022
A Bioactive Janus Barrier Membrane Rejuvenates Bone Regeneration in Aging
1Department of Periodontology & Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Disease & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, Shandong, China.
Abstract:
Aging significantly impairs guided bone regeneration (GBR) by disrupting stem cell recruitment and exacerbating cellular senescence. To address this challenge, we engineered a quercetin and Sr-hydroxyapatite/poly(lactic-co-glycolic acid) (QSHP) Janus barrier membrane. The asymmetric design featured a dense poly(lactic-co-glycolic acid) layer for epithelial exclusion and a functionalized nanowire layer that enabled controlled codelivery of Sr2+ and quercetin. In vitro, the QSHP membrane markedly enhanced the migration and proliferation of bone marrow mesenchymal stem cells while effectively mitigating H2O2-induced senescence and restoring osteogenic capacity. In aged rat mandibular defects, the QSHP membrane treatment yielded the highest newly formed bone volume and restored biomechanical properties approaching those of native bone. Histological and immunofluorescence analyses revealed accelerated early osteogenesis, increased recruitment of CD44+ stem cells, and concomitant attenuation of cellular senescence. Collectively, the QSHP membrane rejuvenates the senescence-impaired bone regenerative niche, enabling functional bone regeneration and offering a clinically promising strategy for GBR in aged patients.
More Related Videos
Related Concept Videos
Bone Remodeling
Tissue Renewal without Stem Cells
However, failure of such a system...
Bone Remodeling and Repair
Osteoclasts in Bone Remodeling
Whole Body Regeneration
Renewal of Skin Epidermal Stem Cells

