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[Biological activity detection of mouse type I soluble interleukin-1 receptor]
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
|November 23, 2001
Abstract
Objective:
To comfirm that the recombinant sIL-1R had biological activity.
Methods:
With biotin labed human IL-1 beta as ligand, protein blotting receptor-ligand binding assay and enzyme-linked immunological receptor-ligand binding assay were developed to detect the expression of sIL-1R I gene in insect cells (Sf9). Meanwhile, the blockage assay of IL-1 beta biological activity was used to detect the function of recombinant sIL-1R I.
Results:
It showed that the recombinant sIL-1R I possessed immunological activity and ligand binding activity and could be secreted into the medium.
Conclusions:
The three assays could be used for detecting biological activity of recombinant sIL-1R I.