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Updated: Oct 3, 2026
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
Published on: August 19, 2014
[Detection of aberrant chromosomes in acute lymphoblastic leukemia by fluorescence in situ hybridization]
Objective:
To investigate the value of fluorescence in situ hybridization (FISH) in detecting aberrant chromosomes in acute lymphoblastic leukemias.
Methods:
Conventional banding techniques and FISH were used to detect trisomy 17, 18 and 21 in 38 patients with acute lymphoblastic leukemia. Lymphoid DNA probes specific for chromosomes no. 17, 18 and 21 were used for FISH.
Results:
Out of the 38 patients, 18 had + 17, 22 + 18 and 23 + 21. The number of trisomy cells ranged from 7% to 80%. Ten normal bone marrow samples were hybridized with the chromosomes 17, 18 and 21 probes.
Conclusion:
FISH is more sensitive than conventional cytogenetic analysis and allows systematic study on large cohort of patients without the need of metaphase preparations.
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