[NMR spin-echo study of the lyophilized biomass of Candida utilis yeasts at different growth stages]

Characteristics of the mobile water fraction isolated from some intracellular structures of freeze-dried cells of Candida utilis were studied as a function of the growth phase by the NMR spin-echo method. Part of the mobile water fraction in the log phase decreases down to ten percent of the biomass weight as compared with 2,5-4% in the stationary phase and in the beginning of the lag phases. Mobility of this water is invariable. A considerable decrease of the mobile water fraction is observed in the post-stationary phase too. A decrease of the mobile water fraction correlates with a decrease of the durability of C. utilis cells and is connected probably with some defects in the membrane structure of the cells and their incomplete development in the log phase of the growth.