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High spatial resolution imaging with near-field scanning optical microscopy in liquids
L F Lee1, R D Schaller, L H Haber
1Department of Chemistry, University of California, Berkeley 94720-1460, USA.
Analytical Chemistry
|November 28, 2001
Abstract:
The mechanism of tuning fork-based shear-force near-field scanning optical microscopy is investigated to determine optimal experimental conditions for imaging soft samples immersed in liquid. High feedback sensitivity and stability are obtained when only the fiber probe, i.e., excluding the tuning fork prongs, is immersed in solution, which also avoids electrical shorting in conductive (i.e., buffer) solutions. Images of MEH-PPV were obtained with comparable spatial resolution in both air and water. High optical resolution (approximately160 nm fwhm) was observed.