Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion.

A S Frangakis1, R Hegerl

  • 1Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, Martinsried, D-82152, Germany.

Journal of Structural Biology
|November 28, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FIB-SEM imaging properties of Drosophila melanogaster tissues embedded in Lowicryl HM20.

Journal of microscopy·2018
Same author

A thin CdSe shell boosts the electron transfer from CdTe quantum dots to methylene blue.

Nanoscale·2018
Same author

Room temperature L1₀ phase transformation in binary CoPt nanostructures prepared by focused-electron-beam-induced deposition.

Nanotechnology·2012
Same author

Marker-free image registration of electron tomography tilt-series.

BMC bioinformatics·2009
Same author

Aberration-corrected microscopy for structural biology applications.

Journal of microscopy·2009
Same author

Fast 3D motif search of EM density maps using a locally normalized cross-correlation function.

Journal of structural biology·2003
Same journal

Ultrastructural evidence of autophagy-related processes and mitochondrial remodeling in the myxozoan parasite Henneguya piaractus.

Journal of structural biology·2026
Same journal

Architecture and dynamics of a supramolecular oxygen transport system in human homogentisate 1,2-Dioxygenase.

Journal of structural biology·2026
Same journal

Connecting pathways between mineralized fibrocartilage and bone at the Achilles tendon insertion.

Journal of structural biology·2026
Same journal

Structural and functional characterization of thermostable EstS1 esterase for BHET degradation.

Journal of structural biology·2026
Same journal

Following the white rabbit: multiscale 2D3D correlative imaging of bone structure.

Journal of structural biology·2026
Same journal

The mantis shrimp eye imaged in 3D using 4th generation synchrotron multiscale phase contrast tomography.

Journal of structural biology·2026
See all related articles

This study introduces a novel noise reduction technique for electron tomography, significantly improving 3D structural visualization of biological specimens by enhancing signal-to-noise ratio while preserving details.

Area of Science:

  • Biophysics
  • Structural Biology
  • Microscopy

Background:

  • Electron tomography (ET) provides 3D structural insights into pleomorphic biological objects.
  • Low signal-to-noise ratio (SNR) in ET data, especially for ice-embedded specimens, hinders visualization and interpretation.
  • Conventional noise reduction methods often compromise signal integrity.

Purpose of the Study:

  • To develop and validate a novel noise reduction technique for ET.
  • To improve the visualization and interpretation of 3D biological structures from noisy ET data.
  • To demonstrate the superiority of the proposed method over existing filtering techniques.

Main Methods:

  • Nonlinear anisotropic diffusion for noise reduction.
  • Fourier shell correlation (FSC) for quantitative SNR assessment.

Related Experiment Videos

  • Application to tomographic reconstructions of chromatin and mitochondria.

    • Main Results:

    • The proposed nonlinear anisotropic diffusion method achieves efficient noise reduction with excellent signal preservation.
    • Demonstrated significant improvement in SNR compared to low-pass, median, and wavelet filtering.
    • Enhanced visualization of 3D structures, including chromatin and mitochondria, after processing.

    Conclusions:

    • Nonlinear anisotropic diffusion is a superior method for noise reduction in electron tomography.
    • This technique significantly enhances the quality of 3D structural data for biological research.
    • The method offers improved visualization capabilities for complex biological specimens.