Studies of a disposable biosensor based on the beta-cyclodextrin inclusion complex as mediator

Area of Science:

• Electrochemistry
• Biosensor Technology
• Biomaterials

Background:

• Development of sensitive and selective electrochemical biosensors is crucial for analyte detection.
• Screen-printed electrodes offer a platform for disposable and cost-effective biosensor fabrication.
• Beta-cyclodextrin inclusion complexes enhance mediator efficiency in electrochemical systems.

Purpose of the Study:

• To develop a disposable voltammetric biosensor for dopamine detection.
• To investigate the role of beta-cyclodextrin inclusion complexes with tetramethylbenzidine and ferrocene as mediators.
• To immobilize mushroom polyphenol oxidase for enhanced biosensor performance.

Main Methods:

• Fabrication of a disposable voltammetric biosensor using a screen-printed basal electrode.
• Immobilization of polyphenol oxidase from mushroom crude extract within a cross-linked beta-cyclodextrin polymer membrane.
• Utilization of beta-cyclodextrin inclusion complexes of tetramethylbenzidine and ferrocene as efficient mediators.
• Electrochemical detection of dopamine using cyclic voltammetry.

Main Results:

• The biosensor demonstrated excellent analytical performance attributed to supramolecular complexation of mediators.
• High selectivity for dopamine detection was observed in the concentration range of 1.0 x 10(-9) to 1.0 x 10(-6) M.
• A low detection limit of 5 x 10(-10) M and a response time under 60 seconds were achieved.
• The biosensor retained its activity for at least 15 days.

Conclusions:

• The developed disposable voltammetric biosensor exhibits superior analytical characteristics for dopamine determination.
• The use of beta-cyclodextrin inclusion complexes significantly enhances biosensor efficiency and selectivity.
• This biosensor represents a promising tool for sensitive and rapid dopamine detection in various applications.