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Detection of ATP competitive protein kinase inhibition by Western blotting

R Wang1, J E Thompson

  • 1Department of Immunology and Rheumatology, Merck Research Laboratories, Rahway, New Jersey 07065, USA.

Analytical Biochemistry
|December 1, 2001
PubMed
Abstract

No abstract available in PubMed .

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.