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Related Experiment Videos

Pattle's bubble-stability method in the rat.

J Havránková, L Kunc, M Kuncová

    Respiration; International Review of Thoracic Diseases
    |January 1, 1975
    PubMed
    Summary
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    Pattle's alveolar bubble-stability method reliably assesses rat surfactant function. Bubble stability is unaffected by illumination or short-term storage but is lower in distilled water than saline.

    Area of Science:

    • Pulmonary physiology
    • Respiratory research
    • Surfactant biophysics

    Background:

    • Surfactant- நுரையீரல் (lung) stability is crucial for respiratory function.
    • Pattle's alveolar bubble method offers a way to study surfactant properties.
    • Understanding factors affecting bubble stability is key for accurate surfactant assessment.

    Purpose of the Study:

    • To evaluate the reliability and influencing factors of Pattle's alveolar bubble-stability method in rats.
    • To determine the impact of storage conditions and fluid type on bubble stability.
    • To provide recommendations for the optimal application of this method in surfactant studies.

    Main Methods:

    • Application of Pattle's alveolar bubble-stability method using lung lobe extracts from rats.

    Related Experiment Videos

  • Comparison of bubble stability ratios in different storage conditions (short-term at 4°C) and fluid environments (distilled water vs. isotonic saline).
  • Assessment of bubble stability irrespective of microscopic illumination and gross pathological lesions.

    • Main Results:

    • No significant difference in bubble stability ratios was observed between separate lung lobes lacking gross pathological lesions.
    • Bubble stability remained consistent despite brief microscopic illumination and 1-day storage at 4°C.
    • A significantly lower stability ratio was recorded in distilled water compared to isotonic saline.

    Conclusions:

    • Pattle's alveolar bubble-stability method demonstrates satisfactory reliability for surfactant studies in rats when measurement timing is strictly controlled.
    • The method's results are not adversely affected by brief illumination or short-term cold storage.
    • The choice of fluid medium (isotonic saline is preferred over distilled water) significantly impacts bubble stability measurements.