Related Experiment Video

Updated: Sep 3, 2026

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
09:16

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

Published on: April 20, 2017

Cellular expression of CCK-A and CCK-B/gastrin receptors in human gastric mucosa

F Schmitz1, M N Göke, J M Otte

  • 1Department of Medicine I, St. Josef-Hospital, Ruhr-University of Bochum, D-44791, Bochum, Germany. frank.schmitz@uni-bochum.de

Regulatory Peptides
|December 4, 2001
PubMed

Insights

Cholecystokinin (CCK) and gastrin receptors are found in human stomach cells, including those regulating acid and somatostatin secretion. This suggests CCK plays a role in maintaining stomach tissue health.

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Cell Biology

Background:

  • Gastrin stimulates gastric acid secretion, but the role of cholecystokinin (CCK) in human gastric acid regulation is debated.
  • Species-specific differences in CCK receptor function and expression are known.

Purpose of the Study:

  • To identify the specific cells in the human gastric mucosa that express CCK and gastrin receptors.
  • To elucidate the cellular targets of CCK and gastrin in the human stomach.

Main Methods:

  • Gastric biopsies from 15 healthy individuals were analyzed.
  • Reverse transcription (RT)-PCR in situ, immunohistochemistry, and confocal laser scanning microscopy were used.
  • Detection of CCK-A and CCK-B/gastrin receptor subtypes at mRNA and protein levels.

Main Results:

  • Both CCK-A and CCK-B/gastrin receptors were found in antral and oxyntic mucosa.
  • CCK-A receptors were located on D cells (producing somatostatin), chief cells, mucous neck cells, and parietal cells in the fundic mucosa.
  • CCK-B/gastrin receptors were identified in parietal cells, neuroendocrine cells (chromogranin A-positive), and epithelial cells in the gastric gland neck.

Conclusions:

  • CCK-A receptors on human D cells provide a basis for CCK's action on somatostatin release.
  • The presence of CCK receptor subtypes in the gastric stem cell compartment suggests a role for CCK in human gastric mucosa homeostasis.

More Related Videos

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization08:16

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

Published on: September 20, 2021

Enhanced Spatial Mapping of Mouse Gastric Muscle Layers Using a Modified Swiss Roll Technique04:18

Enhanced Spatial Mapping of Mouse Gastric Muscle Layers Using a Modified Swiss Roll Technique

Published on: November 25, 2025

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of cells.
Two...
Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical, 7TM, or...
Pathophysiology of Peptic Ulcer Disease: Injurious Factors01:22

Pathophysiology of Peptic Ulcer Disease: Injurious Factors

Peptic ulcers are sores on the stomach's inner lining and the upper small intestine, which are the result of disruptions in the mucosal layer that houses parietal cells which produce gastric acid, and chief cells which secrete pepsinogen.
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds to M3...
Mucosal Barrier of the Stomach01:25

Mucosal Barrier of the Stomach

The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
Gastritis II: Pathophysiology01:26

Gastritis II: Pathophysiology

The pathophysiology of gastritis begins with the colonization of the stomach lining by Helicobacter pylori (H. pylori). This bacterium spreads mainly via the oral-oral route through saliva or shared utensils, and can also be transmitted in overcrowded or unhygienic environments through contaminated water, despite its brief survival outside the body.ColonizationOnce ingested, H. pylori enters the stomach and begins colonization by navigating through the mucus layer lining the stomach wall. It...