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FAM deubiquitylating enzyme is essential for preimplantation mouse embryo development
M Pantaleon1, M Kanai-Azuma, J S Mattick
1Department of Physiology and Pharmacology, The University of Queensland, Queensland 4072, Brisbane, Australia
Mechanisms of Development
|December 4, 2001
Summary
The deubiquitylating enzyme FAM is crucial for early mouse embryo development. Its depletion impairs embryo growth by affecting cell adhesion and regulating beta-catenin and AF-6 protein levels.
Area of Science:
- Developmental Biology
- Molecular Biology
- Cell Biology
Background:
- FAM is a deubiquitylating enzyme that regulates cell adhesion and signaling molecules beta-catenin and AF-6 in vitro.
- Its role in the earliest stages of mammalian development is not fully understood.
Purpose of the Study:
- To investigate the expression and function of FAM during preimplantation mouse embryo development.
- To determine if FAM is essential for early embryonic development and its regulation of key proteins.
Main Methods:
- FAM expression analysis in preimplantation mouse embryos.
- FAM protein depletion using antisense oligodeoxynucleotides in two-cell embryos.
- Assessment of embryo development, cleavage rates, cell adhesion, and protein localization (beta-catenin, AF-6).
Main Results:
- FAM is expressed throughout preimplantation development.
- FAM depletion via antisense oligonucleotides inhibited blastocyst development.
- Loss of FAM decreased cleavage rates, inhibited cell adhesion, and led to beta-catenin loss.
- AF-6 levels initially decreased but normalized, though its localization was altered.
Conclusions:
- FAM is required for successful preimplantation mouse embryo development.
- FAM regulates beta-catenin and AF-6 protein stability and localization in vivo.
- FAM plays a critical role in establishing cell adhesion and proper embryonic development.