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Published on: September 21, 2017
2-Amino-7-deazaadenine forms stable base pairs with cytosine and thymine
Akimitsu Okamoto1, Kazuo Tanaka, Isao Saito
1Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, and CREST, Japan.
Abstract:
2-Amino-7-deazaadenine ((AD)A) was incorporated into oligodeoxynucleotides (ODN) and their base-pairing properties with natural nucleobases were investigated. In melting temperature (T(m)) experiments, the duplex containing an (AD)A/C base pair showed a high stability comparable to that containing (AD)A/T base pair. Destabilization of the duplex usually observed for existing degenerate bases was not observed. However, the incorporation efficiency of dCTP was only 1.8% for TTP in single-nucleotide insertion reactions using polymerase.
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