Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular basis for interaction between Icap1 alpha PTB domain and beta 1 integrin.

David D Chang1, Bao Q Hoang, Jenny Liu

  • 1Department of Medicine, Microbiology, Immunology and Molecular Genetics, UCLA School of Medicine, Los Angeles, California 90095, USA. ddchang@mednet.ucla.edu

The Journal of Biological Chemistry
|December 14, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"Now I feel like I can": exploring e-commerce for self-care contraception in Kenya.

Frontiers in global women's health·2026
Same author

Women's experiences with and preferences for contraceptive side effect counseling and management: A qualitative study from Ethiopia and Kenya.

PloS one·2026
Same author

High-throughput machine learning-aided antibody discovery for cell surface antigens.

Cell systems·2026
Same author

How productive emergency physicians work: a qualitative interview study of clinical workflow in the emergency department.

Scandinavian journal of trauma, resuscitation and emergency medicine·2026
Same author

An in-depth interview study of women's perspectives on the effects of contraceptive use on future fertility in Ethiopia and Kenya.

SSM. Qualitative research in health·2026
Same author

Associations between physician work practices and productivity in emergency departments: a systematic review.

European journal of emergency medicine : official journal of the European Society for Emergency Medicine·2026

Icap1 alpha, a phosphotyrosine binding (PTB) domain protein, specifically binds beta(1) integrin. This interaction is mediated by the NPXY motif on beta(1) integrin and a hydrophobic pocket in Icap1 alpha.

Area of Science:

  • Cellular Biology
  • Protein Interactions
  • Structural Biology

Background:

  • Integrins are crucial cell surface receptors involved in cell adhesion and signaling.
  • Icap1 alpha is a protein that interacts with integrin subunits.
  • Understanding these interactions is key to deciphering cellular processes.

Purpose of the Study:

  • To elucidate the molecular mechanism of Icap1 alpha binding to the integrin beta(1) subunit.
  • To identify critical residues involved in this protein-protein interaction.
  • To determine if Icap1 alpha possesses phosphotyrosine binding (PTB) domain characteristics.

Main Methods:

  • Alanine scanning mutagenesis to identify key residues in beta(1) integrin.
  • Structural modeling based on PTB-peptide structures.

Related Experiment Videos

  • Site-directed mutagenesis to probe Icap1 alpha residues involved in binding.

    • Main Results:

    • Val(787), Val(790), and the NPXY motif (residues 792-795) of beta(1) integrin are critical for Icap1 alpha binding.
    • Specific Icap1 alpha residues (Leu(135), Ile(138), Ile(139), Leu(82), Tyr(144)) are essential for the interaction.
    • Structural modeling predicted key residue proximity and interactions.

    Conclusions:

    • Icap1 alpha functions as a PTB domain protein.
    • Icap1 alpha specifically recognizes the NPXY motif of beta(1) integrin.
    • The interaction between Val(787) of beta(1) integrin and a hydrophobic pocket in Icap1 alpha dictates binding specificity.