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A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Published on: March 15, 2016
Effect of lysophosphatidylcholine on expression of vascular endothelial growth factor in ECV304 cells
1Department of Pharmacology, School of Pharmacy, The Second Military Medical University, Shanghai 200433, China.
Aim:
To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304).
Methods:
VEGF protein production from ECV304 cells was determined by enzyme-linked immunosorbent assay (ELISA). VEGF protein and its receptor were also examined by immunohistochemical staining. After culturing for 24 h with LPC, VEGF mRNA expression in ECV304 was examined by in situ hybridization.
Results:
Cultured ECV304 cells could express VEGF receptor and a strong expression of VEGF receptor was induced by LPC 5 mg/L. LPC upregulated VEGF protein secreted from the ECV304 cells in a time- and concentration-dependent manner and LPC also stimulated VEGF mRNA expression in the ECV304 cells.
Conclusion:
LPC could induce a strong expression of VEGF in ECV304 cells.
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