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Updated: Jul 17, 2026

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
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Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

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CTCF, a candidate trans-acting factor for X-inactivation choice

Wendy Chao1, Khanh D Huynh, Rebecca J Spencer

  • 1Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.

Science (New York, N.Y.)
|December 18, 2001
PubMed
Summary

The study identifies CTCF as a key factor in X-chromosome selection during X-inactivation in mammals. CTCF binding sites within the Tsix gene form an epigenetic switch, regulating which X chromosome is silenced.

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