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Updated: Aug 8, 2026
Progenitor-derived Oligodendrocyte Culture System from Human Fetal Brain
Published on: December 20, 2012
AMPA-Induced suppression of oligodendroglial gene expression in neonatal rat brain
1Department of Pediatrics, University of Michigan, Room 8301 MSRB3, Box 0646, 1150 West Medical Center Drive, Ann Arbor, MI 48109-0646, USA.
Abstract:
Oligodendroglia are susceptible to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-mediated excitotoxicity. We hypothesized that analysis of oligodendroglial gene expression would provide a sensitive indicator of acute excitotoxic oligodendroglial injury in the immature brain. AMPA (2 nmol) was injected intracerebroventricularly in 7-day-old rats. Proteolipid protein and myelin basic protein encoding mRNAs were evaluated 24-48 h later by semi-quantitative reverse transcription-polymerase chain reaction assays. AMPA markedly suppressed brain tissue content of oligodendroglial mRNAs.
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