Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantifying adhesive penetration in adhesive/dentin interface using confocal Raman microspectroscopy.

Yong Wang1, Paulette Spencer

  • 1Department of Oral Biology, University of Missouri-Kansas City School of Dentistry, 650 E. 25(th) Street, Kansas City, Missouri 64108, USA.

Journal of Biomedical Materials Research
|December 18, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unifying Environmental Stress Cracking and Mechano-Sorptive Creep Under the Umbrella of Mechano-Sorptive Phenomena.

Biomimetics (Basel, Switzerland)·2026
Same author

Incorporating Carbamate Functionalities in Multifunctional Monomer System Enhances Mechanical Properties of Methacrylate Dental Adhesives.

Polymers·2025
Same author

Genetic disease risks of under-represented founder populations in New York City.

PLoS genetics·2025
Same author

Editorial: Next-generation dental materials engineered for mineralized tissue reconstruction: advances, challenges and opportunities.

Frontiers in dental medicine·2025
Same author

Scale dependent nanomechanical properties of dentin adhesive and adhesive-collagen composite.

Frontiers in dental medicine·2025
Same author

Synergistic enhancement of hydrophobic dental adhesives: autonomous strengthening, polymerization kinetics, and hydrolytic resistance.

Frontiers in dental medicine·2024

Confocal Raman microspectroscopy quantifies adhesive penetration at the adhesive/dentin interface. Different adhesive systems show varying penetration depths, with Scotchbond Multipurpose Plus exhibiting the steepest concentration gradient.

Area of Science:

  • Biomaterials science
  • Dental materials science
  • Analytical chemistry

Background:

  • The adhesive/dentin interface is critical for dental restoration longevity.
  • Understanding adhesive penetration is key to optimizing bonding strategies.
  • Current methods for analyzing this interface have limitations.

Purpose of the Study:

  • To develop a quantitative method using Confocal Raman Microspectroscopy (CRM) for assessing adhesive penetration at the adhesive/dentin interface.
  • To compare the penetration characteristics of three commercial dentin adhesive systems.

Main Methods:

  • Utilized Confocal Raman Microspectroscopy (CRM) mapping on human dentin specimens treated with three adhesive systems (Scotchbond Multipurpose Plus, Single Bond, Primer Bond NT).
  • Collected Raman spectra from model mixtures of adhesive and type I collagen to create calibration curves based on relative band intensities.

Related Experiment Videos

  • Quantified adhesive percentage across the interface by comparing specimen spectra to calibration curves.

    • Main Results:

    • All three adhesive systems demonstrated a gradual decrease in penetration away from the dentin surface.
    • The adhesive concentration gradient varied among systems, ordered as Scotchbond Multipurpose Plus > Single Bond > Primer Bond NT.
    • CRM provided reliable in situ quantitative analysis of adhesive penetration.

    Conclusions:

    • Confocal Raman Microspectroscopy is a valuable tool for the rapid and accurate quantitative assessment of adhesive penetration at the adhesive/dentin interface.
    • Differences in adhesive penetration are linked to adhesive composition and phase behavior.
    • The developed CRM method aids in understanding and optimizing dental adhesive performance.