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The haplotype analyses using multiple markers of the apolipoprotein B gene in patients with coronary artery disease

S H Hong1, J Song, J Q Kim

  • 1Department of Medicine, University of Maryland Medical School, Baltimore, USA.

Journal of Korean Medical Science
|December 19, 2001
PubMed

Insights

Haplotype analysis of the apolipoprotein B (apoB) gene may identify cardiovascular disease (CAD) risk in Korean populations. While individual apoB gene polymorphisms showed no difference, specific haplotypes were significantly associated with CAD.

Area of Science:

  • Genetics
  • Cardiovascular Disease Research
  • Molecular Biology

Background:

  • Elevated low-density lipoprotein (LDL) is a significant risk factor for cardiovascular disease (CVD).
  • Apolipoprotein B (apoB) is a key protein in LDL, crucial for cholesterol homeostasis.
  • Understanding genetic variations in apoB can offer insights into CVD susceptibility.

Purpose of the Study:

  • To investigate the association between apolipoprotein B (apoB) gene polymorphisms and coronary artery disease (CAD) in a Korean population.
  • To compare apoB allelic and haplotype frequencies between CAD patients and healthy controls.
  • To examine the relationship between apoB polymorphisms and plasma lipid levels.

Main Methods:

  • Genotyping of six polymorphic sites within the apoB gene.
  • Analysis of allele and haplotype frequencies in 235 CAD patients and 216 controls.
  • Comparison of allelic distributions with Chinese and Caucasian populations.
  • Correlation analysis with plasma lipid profiles.

Main Results:

  • No significant differences in individual apoB allele frequencies were observed between CAD patients and controls.
  • Haplotype frequencies of the apoB gene showed a significant difference between the patient and control groups (p<0.05).
  • Allelic distributions in Koreans were similar to Chinese but distinct from Caucasians; no association with plasma lipid levels was found.

Conclusions:

  • Haplotype analysis of the apoB gene, utilizing multiple diallelic markers, may serve as a valuable genetic marker for identifying CAD risk in Korean individuals.
  • Individual apoB polymorphisms are not strongly associated with CAD in this population, highlighting the importance of haplotype-based approaches.
  • Genetic variations in apoB show population-specific differences, particularly between East Asian and Caucasian groups.

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