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Related Experiment Videos

Anesthetic effects on mitochondrial ATP-sensitive K channel.

S Kohro1, Q H Hogan, Y Nakae

  • 1Department of Anesthesiology Research, Medical College of Wisconsin, Milwaukee 53226, USA.

Anesthesiology
|December 19, 2001
PubMed
Summary

Inhalational anesthetics like isoflurane and sevoflurane protect the heart by opening mitochondrial K(ATP) channels, increasing flavoprotein oxidation. Intravenous anesthetics may block this protective effect.

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Area of Science:

  • Anesthesiology
  • Cardiology
  • Mitochondrial Physiology

Background:

  • Volatile anesthetics offer cardioprotection mimicking ischemic preconditioning.
  • Intravenous anesthetics also provide protection against ischemic-reperfusion injury.
  • Mitochondrial ATP-regulated potassium (mitoK(ATP)) channels are implicated in cardiac preconditioning, but anesthetic effects on them are unknown.

Purpose of the Study:

  • To investigate if anesthetics affect mitoK(ATP) channels.
  • To determine the impact of anesthetics on mitochondrial flavoprotein oxidation.
  • To explore the role of mitoK(ATP) channels in anesthetic-induced cardioprotection.

Main Methods:

  • Isolated guinea pig myocardial cells were used.
  • Mitochondrial flavoprotein oxidation was measured using fluorescence microscopy.

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  • Cells were exposed to isoflurane, sevoflurane, propofol, and pentobarbital.
  • The effect of a mitoK(ATP) channel antagonist (5-hydroxydeanoate) was assessed.

    • Main Results:

    • Isoflurane and sevoflurane dose-dependently increased flavoprotein oxidation.
    • This effect was blocked by 5-hydroxydeanoate, indicating mitoK(ATP) channel involvement.
    • Propofol and pentobarbital did not affect flavoprotein oxidation but inhibited the effects of isoflurane.

    Conclusions:

    • Inhalational anesthetics activate flavoprotein oxidation via mitoK(ATP) channels, contributing to cardioprotection.
    • Cardioprotective effects of intravenous anesthetics may not involve flavoprotein oxidation.
    • Co-administration of propofol or pentobarbital might diminish the cardioprotective benefits of inhalational anesthetics.