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Motor pudendal nerve characterization in the female rat
Derek D Kane1, Susan Shott, W Frank Hughes
1Department of Anatomy, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612-3832, USA.
The Anatomical Record
|December 19, 2001
Summary
This study quantifies pudendal motor neurons and axons in female rats, finding distinct neuron populations innervate separate sphincters. Pudendal nerve anatomy is characterized, providing a basis for incontinence research.
Area of Science:
- Neuroscience
- Anatomy
Background:
- The pudendal nerve plays a crucial role in pelvic floor function.
- Understanding its anatomy is vital for treating conditions like neurogenic incontinence.
Purpose of the Study:
- To quantitatively characterize pudendal motor neuron cell bodies and myelinated axons in the female rat.
- To establish a baseline for future studies on pudendal nerve injury and repair.
Main Methods:
- Fluorescent retrograde tracers (diamidino yellow, fast blue) were injected into sphincters to label motor neurons.
- Motor neurons were identified and counted in the L6 spinal cord.
- Myelinated axons in the pudendal nerve were counted using light microscopy.
Main Results:
- Distinct populations of motor neurons innervate the external urethral sphincter and external anal sphincter.
- The mean myelinated axon count in the pudendal nerve was 151.4 +/- 17.0.
- Myelinated axons do not branch as the pudendal nerve divides, and the neuron to axon ratio suggests sensory or other motor functions.
Conclusions:
- Pudendal motor neurons are topographically organized, with separate columns innervating distinct sphincters.
- The characterized pudendal nerve anatomy provides a reliable model for studying neurogenic incontinence.