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Blood contamination and dentin bonding--effect of anticoagulant in laboratory studies
Thomas Dietrich1, Martin L J Kraemer, Jean Francois Roulet
1Department of Periodontology and Synoptic Dentistry, Humboldt-University, Charité, Berlin, Germany. tommi@tomsoft.com
Objective:
To investigate the influence of heparinized blood vs. native blood on the marginal adaptation of dentin bonded composite resin restorations.
Methods:
Cylindrical dentin cavities were prepared in 40 extracted human incisors. After acid-etching four groups (n=10) were contaminated with either fresh capillary blood (FCB), heparinized venous blood (HPB), saline (SAL) or heparinized saline (HPS) and bonded and filled with Scotchbond 1/Z 100. After water storage for 21 days and thermocycling (2000 x, 5-55 degrees C), replicas were produced for evaluation of marginal adaptation by means of quantitative marginal analysis in the SEM. Statistical analysis was performed with the Kruskal-Wallis-test and the Bonferroni correction for multiple comparisons at p<0.05.
Results:
There were no differences between groups SAL, HPS and HPB. Contamination with fresh capillary blood resulted in significantly higher amounts of marginal openings compared to all other groups.
Significance:
Freshly drawn blood has to be used for blood contamination experiments in laboratory studies.