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Molecular characterization of beta-tubulin gene from Pleurotus sajor-caju
1Division of Applied Microbiology, National Institute of Agricultural Science and Technology, Suweon, Korea. bgkimyes@rda.go.kr
Bioscience, Biotechnology, and Biochemistry
|January 5, 2002
Abstract:
A beta-tubulin gene (TUB1) from the basidiomycete Pleurotus sajor-caju was sequenced. TUB1 encodes a 446-amino-acid protein. The coding region is interrupted by 9 introns, all of which had a 5'-GTRNGT... YAG-3' sequence at the boundaries. Locations of the introns in TUB1 were common between the beta-tubulin genes of other basidiomycetes, but not with animals, ascomycetes, or plants. This suggests that the introns were inserted independently into the beta-tubulin gene after these divisions had diverged.