Application of the GPT system in paternity cases

Area of Science:

• Biochemistry
• Genetics
• Forensic Science

Background:

• Glutamic-pyruvic transaminase (GPT) is an enzyme with polymorphic isoenzymes.
• GPT isoenzyme patterns can be affected by blood storage conditions.
• Understanding GPT stability is essential for its reliable use in genetic analysis.

Purpose of the Study:

• To assess the reproducibility and stability of GPT starch gel patterns.
• To determine the influence of storage conditions on GPT isoenzyme patterns.
• To evaluate the utility of the GPT system in paternity testing.

Main Methods:

• GPT starch gel electrophoresis was used to analyze blood samples.
• Reproducibility of GPT patterns was tested.
• The effect of storage duration and temperature on haemolysates was investigated.
• Statistical analysis was performed on paternity cases.

Main Results:

• GPT starch gel patterns are fully reproducible and constant after one month of age.
• Unfrozen blood GPT patterns change during storage; samples should be processed within 4 days.
• GPT isoenzyme patterns remain stable for months when haemolysates are stored at -25°C.
• The GPT system provides an 18.7% chance of paternity exclusion.

Conclusions:

• The GPT system is a reliable genetic marker for individuals over one month old.
• Standardized blood sample handling and storage protocols are vital for accurate GPT analysis.
• The GPT system significantly enhances the statistical power of genetic evidence in disputed paternity cases.