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Qualitative and quantitative liquid chromatographic determination of natural retinoids in biological samples
1Institute for Nutrition Research, Faculty of Medicine, University of Oslo, Blindern, Norway. thomasgu@basalmed.uio.no
Abstract:
Liquid chromatography continues to be the preferred method for determining retinoids in biological samples. The highly unstable nature of retinoids and the real possibility of artifacts or erroneous results have led to the development of rapid and highly automated protocols for retinoid extraction, separation and detection. Due to strong light absorbance in the ultraviolet region, UV detectors still predominate although mass spectrometric detection is gaining increased popularity. This paper reviews recent advances and provides major guidelines for using liquid chromatography to identify and quantify retinoids in biological samples.