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Updated: Apr 23, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

High-level multiplex DNA amplification.

N E Broude¹, K Driscoll, C R Cantor

¹Center for Advanced Biotechnology and Department of Biomedical Engineering, Boston University, MA 02215, USA. nebroude@hotmail.com

Antisense & Nucleic Acid Drug Development

|January 5, 2002

Summary

This summary is machine-generated.

This study enhances multiplexed polymerase chain reaction (PCR) for amplifying many DNA targets in one tube. The optimized method efficiently amplifies 30 DNA targets, including single nucleotide polymorphisms (SNPs), for diverse applications.

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Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

Area of Science:

Molecular Biology
Genetics

Background:

Multiplexed polymerase chain reaction (PCR) enables simultaneous amplification of multiple DNA targets.
Existing methods can be limited in the number of targets amplified simultaneously.

Purpose of the Study:

To present an enhanced single-tube multiplexed PCR method for efficient amplification of a large number of DNA targets.
To optimize reaction conditions for simultaneous synthesis of 30 DNA targets.

Main Methods:

Utilized a PCR suppression-based approach with one sequence-specific primer per amplicon and a common second primer for all targets.
Optimized reaction conditions for simultaneous amplification of 30 DNA targets, including single nucleotide polymorphisms (SNPs).

Main Results:

Demonstrated efficient amplification of up to 30 DNA targets in a single PCR tube.
Amplified fragments varied in size from 100 to 600 bp and were derived from multiple human chromosomes.
Successfully amplified fragments containing single nucleotide polymorphisms (SNPs).

Conclusions:

The developed method offers highly multiplexed DNA amplification capabilities.
This technique has significant potential for applications in SNP analysis, DNA diagnostics, and forensic science.