Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An artificial primosome: design, function, and applications.

V V Demidov1, N E Broude, I V Lavrentieva-Smolina

  • 1Center for Advanced Biotechnology, Department of Biomedical Engineering, Boston University, 36 Cummington Street, Boston, MA 02215, USA. vvd@bu.edu

Chembiochem : a European Journal of Chemical Biology
|February 6, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determinants of umbilical cord arterial 8-iso-prostaglandin F2alpha concentrations.

BJOG : an international journal of obstetrics and gynaecology·2000
Same author

A sensitive gas chromatography-mass spectrometry assay reveals increased levels of monohydroxyeicosatetraenoic acid isomers in human plasma after extracorporeal photoimmunotherapy and under in vitro ultraviolet A exposure.

The Journal of investigative dermatology·2000
Same author

[Transcoronary ablation of septal hypertrophy (TASH): a 5-year experience].

Zeitschrift fur Kardiologie·2000
Same author

Bacterial and nonbacterial expression of wild-type and mutant human phospholipid hydroperoxide glutathione peroxidase and purification of the mutant enzyme in the milligram scale.

Protein expression and purification·2000
Same author

Multilocular inoculation tuberculosis of the skin after stay in Africa: detection of mycobacterial DNA using polymerase chain reaction.

The British journal of dermatology·2000
Same author

Transcoronary ablation of septal hypertrophy (TASH): a new treatment option for hypertrophic obstructive cardiomyopathy.

Zeitschrift fur Kardiologie·2000
Same journal

Reaction Optimization for Enzymatic Deconstruction of Industrially Relevant Nylon Composites.

Chembiochem : a European journal of chemical biology·2026
Same journal

Deploying Artificial Metalloenzymes in Complex Environments: Strategies and Applications.

Chembiochem : a European journal of chemical biology·2026
Same journal

Synthetic Ligands of Myeloid C-Type Lectin Receptors.

Chembiochem : a European journal of chemical biology·2026
Same journal

Vancomycin-Mediated Binding of DNA Origami Nanostructures to Gram-Positive and Gram-Negative Bacteria.

Chembiochem : a European journal of chemical biology·2026
Same journal

Mutasynthesis and Antibiotic Activity of Mupirocin Analogues.

Chembiochem : a European journal of chemical biology·2026
Same journal

Pressure-Dependent Aromatic Ring Flips Reveal Variable Transition-State Volume and Compressibility Among Structural Regions of BPTI.

Chembiochem : a European journal of chemical biology·2026
See all related articles

Peptide nucleic acid (PNA)-assisted PD-loops act as artificial primosomes, enabling DNA polymerase to initiate primer extension on double-stranded DNA (dsDNA). This breakthrough offers efficient DNA priming for applications in diagnostics and sequencing.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Biotechnology

Background:

  • Double-stranded DNA (dsDNA) priming typically requires single-stranded DNA templates.
  • Existing methods for DNA manipulation can be complex and require extensive purification.

Purpose of the Study:

  • To investigate the potential of peptide nucleic acid (PNA)-assisted PD-loops as artificial primosomes for dsDNA.
  • To evaluate the efficiency and applications of this novel priming mechanism.

Main Methods:

  • Formation of PD-loops using PNA and oligodeoxyribonucleotides on linear dsDNA.
  • Initiation of primer extension by DNA polymerase with strand displacement activity.
  • Assessment of priming efficiency compared to traditional methods.

Related Experiment Videos

Main Results:

  • PD-loops effectively function as artificial primosomes on linear, nonsupercoiled dsDNA.
  • Primer extension by DNA polymerase occurs at designated dsDNA sites, yielding extended primers.
  • The efficiency of dsDNA priming by PD-loops is comparable to single-stranded DNA priming.

Conclusions:

  • The PD-loop structure serves as a functional artificial primosome for dsDNA.
  • This technology has potential applications in DNA diagnostics, including ultrasensitive quantification and nondenaturing sequencing.
  • The method allows for direct isothermal DNA sequencing without prior purification of the target DNA.