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Updated: Aug 7, 2026

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Published on: September 13, 2013
Reporter-gene systems for the study of G-protein-coupled receptors
1Institute of Cell Signalling, Medical School, University of Nottingham, Queen's Medical Centre, UK. stephen.hill@nottingham.ac.uk
Abstract:
Reporter-gene assays offer an alternative to biochemical assays for following signal transduction pathways from receptors at the cell surface to nuclear gene transcription in living cells. Specific reporter-gene systems are now available for the study of ligand activity at G alpha(i/o), G alpha(s) and G alpha(q) G-protein-coupled receptors. In recent years reporter genes have been applied in academia and industry to the study of ligand efficacy and affinity in recombinant and primary cell lines using a variety of colour, fluorescent or luminescent read-outs.
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