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Updated: Aug 9, 2026
Localization, Identification, and Excision of Murine Adipose Depots
Published on: December 4, 2014
[Experimental comparison of the adipocytal injuries harvested by three surgical methods]
1Department of Burn and Plastic Surgery, 174 Hospital of PLA, Xiamen 361003, China.
Objective:
To evaluate the degree of the injuries on the fat cells harvested by cutting, syringe aspiration and pipe suction techniques.
Methods:
Fat tissue was removed from lower abdomen by cutting, syringe aspiration and pipe suction. The excised adipose tissue was cut into 3 mm diameter fat particles, whereas the adipose tissue from syringe aspiration group was taken by the syringe suction technique with No. 22 needle and in the pipe suction group the adipose tissue from the suction tube with 8 mm in diameter. Each sample was fixed in 10% buffered formalin and stained with hematoxylin technique and wilder's silver technique. The fat cells were evaluated under microscope.
Results:
The percentage of the injured fat cells was (8.6 +/- 1.8)% in cutting technique, (15.7 +/- 2.4)% in syringe aspiration and (76.4 +/- 8.7)% in pipe suction respectively. They had significant differences between each other.
Conclusions:
Among the three methods, the degree of the injuries on the fat cells was mostly serious in the way of pipe suction technique while minimal in the way by cutting.
